|
KMID : 1145120180160010042
|
|
Asian Journal of Beauty and Cosmetology
2018 Volume.16 No. 1 p.42 ~ p.51
|
|
|
Human Dermal Stem/Progenitor Cell-derived Conditioned Medium Ameliorates Ultraviolet A-induced Damage of Normal Human Epidermal Keratinocytes
|
|
Shim Joong-Hyun
|
|
|
Abstract
|
|
|
Purpose: The present study aimed to define the epidermal moisturizing effects of human dermal stem/progenitor cell-derived conditioned medium (hDSPC-CM) on normal human epidermal keratinocytes (NHEKs).
Methods: To investigate the epidermal moisturizing effects of hDSPC-CM on NHEKs, I measured 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and gene expression and performed cell viability assay and hyaluronic acid (HA)?enzyme-linked immunosorbent assay (ELISA).
Results: In the present study, I elucidated the effects of hDSPC-CM on the mRNA expression of hyaluronan synthase 2 (HAS2), aquaporin 3 (AQP3), keratin 1 (KRT1), and keratin 10 (KRT10) and HA production. Real-time polymerase chain reaction (real-time PCR) revealed that hDSPC-CM increased HAS2 and AQP3 expression in NHEKs, and HA-ELISA revealed that hDSPC-CM increased HA production in NHEKs. On the other hand, hDSPC-CM decreased KRT1 and KRT10 expression in NHEKs.
Conclusion: The results revealed that hDSPC-CM is a potent moisturizing cosmetic ingredient for epidermal moisturization. Based on this study, I anticipate further studies on mechanisms underlying the effects of hDSPC-CM on epidermal keratinocytes to develop not only cosmetics but also healthcare medicines.
|
|
|
KEYWORD
|
|
|
hDSPC-CM, Moisturizing, HAS2, AQP3, Hyaluronic acid
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|